Muhammad Awais Zafar 1, Farah Ali *2, Laeeq Akbar Lodhi1, Syed Saad Ul Hassan1, Athar Ilyas1, Muhammad Shoaib Iqbal 1, Ali Zahid1, Riaz Hussain 2 and Muhammad Saqib Saeed1
1Department of Theriogenology, University of Agriculture, Faisalabad-38040, Pakistan 2University College of Veterinary and Animal Sciences, The Islamia University Bahawalpur, Pakistan
*Corresponding author: drfarahiub@gmail.com
The aim of this study was to determine the effect of tocopherol and selenium on the performance of buserelin (GnRH analogue) for estrus induction in anestrus mares. A total of 18 seasonally anestrus mares were divided into three equal groups (n=6 in each) after ovarian scanning. Mares in group A (treatment group) were treated with tocopherol and selenium intramuscularly along with daily dose of buserelin. Mares which came into heat within ten-day buserelin protocol, further injections of buserelin for those mares were stopped. Mares in group B were treated with buserelin intramuscularly only for ten days. Mares which came into heat within ten-day buserelin protocol, further injections of buserelin were stopped. Blood/serum was collected from each experimental mare kept in different groups (A-C) at days 1, 11, 12, 13 and 14. Onset of estrus signs was 83.3, 33.3 and 0% in group A, B and C, respectively, that was significant (P<0.05) among groups. Follicular dynamics were 20.24±3.00, 14.59±2.24 and 10.35±0.63mm in groups A, B and C, respectively with significant (P<0.05) difference. Change in serum E2 contents was significantly (P<0.05) different among groups A, B and C whereas progesterone concentration showed non-significant difference among groups.