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Antimicrobial resistance represents one of the major global health challenges. Consequently, the efficacy of conventional antimicrobials has been progressively reduced by the emergence of multidrug-resistant strains, making the search for alternative antimicrobials essential. This study aimed to evaluate the antimicrobial activity of (R)-(+)-limonene against strains of Pseudomonas aeruginosa isolated from food and to investigate its possible mechanism of action via molecular docking with DNA gyrase B. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) techniques were performed using microdilution in 96-well plates, whilst the Minimum Inhibitory Concentration for Adhesion (MICA) was assessed in test tubes. Additionally, interaction with antimicrobials was investigated using the disc diffusion method, and molecular docking was performed using AutoDock 4.2 software. The results showed an MIC90 of 1000 ?g/mL and a MBC at ratios of 1:1 and 2:1, indicating a bactericidal effect. With regard to anti-adhesive activity, (R)-(+)-limonene inhibited biofilm formation up to a ratio of 1:8. Regarding combination with antimicrobials, a synergistic effect was observed with ampicillin, gentamicin, tetracycline, ciprofloxacin, ceftazidime and cefazolin. Additionally, interactions with the active site of the DNA gyrase B enzyme were identified, mainly via hydrophobic forces, van der Waals forces and alkyl/pi-alkyl interactions. These findings reinforce the potential of (R)-(+)-limonene as a promising agent for microbial control, as well as highlighting the relevance of bioact